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 The horizontal axis represents the H3 sequence and the vertical axis represents the amino acids by which the corresponding row is mutated.
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 The value of creating in depth, tissue-specific maps of protein lysine acetylation websites is additional underscored by the numerous function of posttranslational modifications in the evolution and phenotypic composition of vertebrates, as indicated by the significant selective stress gene regulatory elements near posttranslational-modifying enzymes have come beneath in placental animals ( Lowe et al., 2011 ).
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 Since totally different morphology of cells of epithelial type is observed in two varieties of tissue, the share of immunoractive cells (labelling index) was calculated within the consultant pictures of each pattern and used for the comparison of the two sorts of colorectal tissue.
 For additional validation, we chose histone H3 lysine 27 acetylation as a result of this modification has not been previously proven to be altered in CRC 19Protein immunostaining on western blots confirmed the increased K27 acetylation at H3 (Figure 3 ) and immunohistochemical staining of paired CRC and corresponding normal tissue sections revealed nuclear localisation of acetylated H3 protein (Figure 4 ).
 A) Discrimination components of NSD1 for the recognition of amino acid residues on the positions next to the target lysine (K36 within the experiment shown here).
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